Abnormally High pKa of an Active-Site Glutamic Acid Residue in Bacillus Circulans Xylanase
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چکیده
منابع مشابه
Evidence that glutamic acid 167 is an active-site residue of Shiga-like toxin I.
Escherichia coli Shiga-like toxin I, a close relative of Shiga toxin and a distant relative of the ricin family of plant toxins, inhibits eukaryotic protein synthesis by catalyzing the depurination of adenosine 4324 in 28S rRNA. By comparing the crystallographic structure of ricin with amino acids conserved between the Shiga and ricin toxin families, we identified seven potential active-site re...
متن کاملCharacterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.
Bacillus circulans xylanase contains two histidines, one of which (His 156) is solvent exposed, whereas the other (His 149) is buried within its hydrophobic core. His 149 is involved in a network of hydrogen bonds with an internal water and Ser 130, as well as a potential weak aromatic-aromatic interaction with Tyr 105. These three residues, and their network of interactions with the bound wate...
متن کاملA glutamic acid-producing bacillus.
During ail examination of microbial culture filtrates capable of reversing the inhibition of Streptomyces aureofaciens Strain NRRL B-1286 caused by the metliionine analogue norleucine, one filtrate was found to possess striking reversing potency. Analysis showed this could not be due to methionine. The reversal properties of the filtrate were traced to glutamic acid which imparted high reversin...
متن کاملHyperexpression of a Bacillus circulans xylanase gene in Escherichia coli and characterization of the gene product.
A 4.0-kilobase (kb) fragment of Bacillus circulans genomic DNA inserted into pUC19 and encoding endoxylanase activity was subjected to a series of subclonings. A 1.0-kb HindIII-HincII subfragment was found to code for xylanase activity. Maximum expression levels were observed with a subclone that contained an additional 0.3-kb sequence upstream from the coding region. Enhancer sequences in the ...
متن کاملMapping of residues involved in the interaction between the Bacillus subtilis xylanase A and proteinaceous wheat xylanase inhibitors.
The Bacillus subtilis xylanase A was subjected to site-directed mutagenesis, aimed at changing the interaction with Triticum aestivum xylanase inhibitor, the only wheat endogenous proteinaceous xylanase inhibitor interacting with this xylanase. The published structure of Bacillus circulans XynA was used to target amino acids surrounding the active site cleft of B.subtilis XynA for mutation. Twe...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2008
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1995.0839a.x